# DBT JRF 2020 — Question 19

Exam: DBT JRF 2020 · Section: A · Topic: Biotechnology · Difficulty: Easy

## Question

The enzyme used in glucometers to estimate blood glucose levels is

## Options

- **A.** glucose isomerase
- **B.** glucose oxidase
- **C.** hexokinase
- **D.** insulin

## Answer

Option B

## Explanation

Glucose oxidase (GOx) catalyses the reaction: $\text{Glucose} + \text{O}_2 \rightarrow \text{Glucono-}\delta\text{-lactone} + \text{H}_2\text{O}_2$ . The $\text{H}_2\text{O}_2$ generated is proportional to glucose concentration. In electrochemical glucometers, $\text{H}_2\text{O}_2$ is oxidised at the electrode surface at +0.6 V (vs. Ag/AgCl), generating an electric current that is measured and converted to a glucose reading. GOx is specific, sensitive, and robust — ideal for point-of-care diagnostics. Hexokinase is used in clinical laboratory glucose assays but not in hand-held glucometers.

Source: https://mypyqbuddy.com/questions/the-enzyme-used-in-glucometers-to-estimate-blood-6a05a8a02adafec4702931f9