# DBT JRF 2020 — Question 62

Exam: DBT JRF 2020 · Section: B · Topic: Microbiology · Difficulty: Medium

## Question

The malarial parasite that has caused recent outbreaks of Monkey malaria in humans is

## Options

- **A.** Plasmodium berghei
- **B.** Plasmodium vivax
- **C.** Plasmodium knowlesi
- **D.** Plasmodium malariae

## Answer

Option C

## Explanation

Bacterial (prokaryotic) DNA replication features: (1) Semi-conservative — each daughter cell receives one parental and one newly synthesised strand (Meselson-Stahl, 1958). (2) Semi-discontinuous — leading strand synthesised continuously, lagging strand synthesised as Okazaki fragments (1–2 kb in bacteria). (3) Chain growth 5'→3' — DNA polymerase can only add nucleotides to a free 3'-OH. (4) BIDIRECTIONAL (NOT unidirectional) — two replication forks travel in OPPOSITE directions from the single origin (oriC) in E. coli. Each fork is replicated by a replisome (DnaB helicase, primase DnaG, DNA Pol III holoenzyme). Replication terminates at Ter sequences where Tus protein blocks fork progression. Eukaryotic replication is also bidirectional from multiple origins.

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