# DBT JRF 2022 — Question 33

Exam: DBT JRF 2022 · Section: B · Topic: Molecular Biology › DNA Fingerprinting & Polymorphism Markers · Difficulty: Medium

## Question

Which one of the following markers is NOT routinely used for DNA fingerprinting?

## Options

- **A.** Amplification short tandem repeats
- **B.** Analysis of copy number variations
- **C.** Restriction fragment length polymorphism
- **D.** Amplification variable number tandem repeats

## Answer

Option B

## Explanation

DNA fingerprinting techniques: Standard DNA fingerprinting methods include: • STR (Short Tandem Repeat) amplification: PCR amplification of microsatellite loci — most widely used (forensics, paternity) • RFLP (Restriction Fragment Length Polymorphism): digestion with restriction enzymes → Southern blot — classic method • VNTR (Variable Number Tandem Repeats): larger tandem repeats detected by Southern blotting — original 'DNA fingerprinting' by Jeffreys NOT a routine DNA fingerprinting technique: • Copy Number Variation (CNV) analysis: CNVs are large genomic segments present in different copy numbers between individuals. CNV analysis is used in research and clinical diagnostics (e.g., cancer, autism) but is NOT part of standard DNA fingerprinting protocols for identity testing. Answer: Analysis of copy number variations (CNVs) (identified in the question options).

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