# DBT JRF 2022 — Question 41

Exam: DBT JRF 2022 · Section: B · Topic: Bioinformatics › Multiple Sequence Alignment Tools · Difficulty: Medium

## Question

Which one of the following CANNOT be used for progressive global multiple sequence alignment?

## Options

- **A.** ClustalW
- **B.** LALIGN
- **C.** PILEUP
- **D.** T-COFFEE

## Answer

Option B

## Explanation

LALIGN and multiple sequence alignment: Bioinformatics tools for sequence alignment: • LALIGN: Finds multiple LOCAL alignments between TWO sequences (pairwise). Based on Smith-Waterman algorithm. Identifies non-overlapping similar regions within the same pair. NOT for multiple sequence alignment. • ClustalW: Progressive global multiple sequence alignment of protein or DNA sequences. • PILEUP (GCG): Progressive multiple sequence alignment tool. • T-COFFEE: Multiple sequence alignment that uses pairwise alignments to guide progressive alignment; generally more accurate than ClustalW. The tool NOT used for progressive global multiple sequence alignment is LALIGN. Answer: LALIGN (it performs pairwise local alignment, not progressive multiple sequence alignment).

Source: https://mypyqbuddy.com/questions/which-one-of-the-following-cannot-be-used-6a104cd5920734408bc0c738