# DBT JRF 2022 — Question 138

Exam: DBT JRF 2022 · Section: B · Topic: Animal Biotechnology › Animal Cloning - Somatic Cell Nuclear Transfer · Difficulty: Medium

## Question

The technique used in animal biotechnology for rapid multiplication and production of animals with a desired genotype is:

## Options

- **A.** Protoplast fusion and embryo transfer
- **B.** Hybrid selection and embryo transfer
- **C.** In vitro fertilization and embryo transfer
- **D.** Nuclear injection of genetic material and embryo transfer

## Answer

Option C

## Explanation

In vitro fertilisation and embryo transfer for animal multiplication: Animal biotechnology techniques for rapid multiplication of genetically superior animals: • IVF + Embryo Transfer procedure: 1. SUPEROVULATION: Donor female is treated with gonadotropins (FSH) to stimulate multiple ovulations (10–20 eggs instead of 1). 2. IVF: Oocytes are harvested and fertilised in vitro with selected semen. 3. EMBRYO CULTURE: Embryos are cultured to 8-cell or blastocyst stage. 4. EMBRYO TRANSFER: Embryos are transferred to synchronised surrogate mothers. • This allows one high-value female to produce many more offspring than naturally possible. • Combines with cryopreservation of embryos for storage and international transport. • Multiple Ovulation Embryo Transfer (MOET) is widely used in cattle breeding. Answer: IVF + Embryo Transfer allows rapid multiplication of desired genotype by using surrogate mothers to gestate multiple embryos from a superior donor female.

Source: https://mypyqbuddy.com/questions/the-technique-used-in-animal-biotechnology-for-rapid-6a104cd5920734408bc0c799