Consider a bacterium that grows with doubling time of 20 min in the exponential phase of its growth cycle and acquire 10 random mutations in its…
Consider a bacterium that grows with doubling time of 20 min in the exponential phase of its growth cycle and acquire 10 random mutations in its genome in every generation. How many mutations will it acquire after 48 hr of growth in exponential phase?
Select an option to attempt
Correct Answer: Option C
Explanation
In a constant-volume fed-batch culture (substrate fed at constant ratewith feed concentration): The substrate balance is. As culture proceeds, cell biomassincreases (more cells competing for the same substrate feed rate). With fixedand, the substrate concentration per cell decreases. From Monod kinetics:— asdecreases (because substrate is consumed faster by the growing biomass),decreases. Decliningwith increasing biomass is the hallmark of non-exponential growth in fed-batch cultures.