During genome engineering process, the role of Flippase enzyme in the next round of modification in the target gene is to
During genome engineering process, the role of Flippase enzyme in the next round of modification in the target gene is to
Select an option to attempt
Correct Answer: Option A
Explanation
Flippase recombinase (FLP recombinase from Saccharomyces cerevisiae 2μ plasmid) catalyses site-specific recombination between two FRT (Flippase Recognition Target) sequences. In the Cre-lox/FLP-FRT system used for genome engineering: Step 1 — An antibiotic resistance cassette (for selection) flanked by two FRT sites is knocked into the target gene. Step 2 — After selection confirms successful targeting, FLP recombinase is expressed, catalysing recombination between the two FRT sites — excising and REMOVING the antibiotic cassette. This leaves a single FRT 'scar' and a clean locus ready for the next round of modification. Without FLP removal of the antibiotic marker, the same marker cannot be used again in subsequent modifications.