For monoclonal antibody production, mammalian expression systems are preferred over bacterial expression systems, primarily because:
For monoclonal antibody production, mammalian expression systems are preferred over bacterial expression systems, primarily because:
Select an option to attempt
Correct Answer: Option C
Explanation
Monoclonal antibody production — mammalian cells vs. bacteria:Monoclonal antibodies (mAbs) are produced in MAMMALIAN CELL LINES (CHO, NSO, HEK293) rather than bacteria for a critical reason: GLYCOSYLATION.• IgG antibodies require N-linked glycosylation at Asn297 in the Fc region (CH2 domain).• This glycosylation is essential for:- ADCC (antibody-dependent cellular cytotoxicity)- CDC (complement-dependent cytotoxicity)- FcRn-mediated recycling (determines half-life ~21 days)- Protein stability and solubility• Bacteria: lack the glycosylation machinery → produce aglycosylated antibodies → reduced efficacy, altered pharmacokinetics, potential immunogenicity.• Mammalian cells: provide human-like complex N-glycans → proper effector functions.Note: Engineering yeast and plant systems for 'humanised' glycosylation is an active research area.Answer: Mammalian cells are needed for proper glycosylation of antibody Fc region; bacteria cannot glycosylate proteins.