A buffer contains 10% glucose, 20 mM Tris, and 50 mM HCl. For making 1 litre of buffer from the following stock solutions – 50% glucose, 1 M Tris,…
A buffer contains 10% glucose, 20 mM Tris, and 50 mM HCl. For making 1 litre of buffer from the following stock solutions – 50% glucose, 1 M Tris, and 1 M HCl, the correct combination of volume of each of the stock solutions will be
Select an option to attempt
Correct Answer: Option A
Explanation
Using the dilution formulafor each component to prepare 1 L of buffer: Glucose:,,→. Tris:,,→. HCl:,,→. Answer: 200 mL glucose + 20 mL Tris + 50 mL HCl, made up to 1 L with water.