A laboratory is trying to purify a Histone-binding protein from its native source. Which one of the following purification techniques should be used…
A laboratory is trying to purify a Histone-binding protein from its native source. Which one of the following purification techniques should be used for one-step purification of the protein?
Select an option to attempt
Correct Answer: Option B
Explanation
One-step purification of a histone-binding protein:For one-step purification of a protein that binds histones:• Immuno-affinity chromatography: Use a column with immobilised anti-histone antibodies (or immobilised histones). The histone-binding protein will bind specifically and elute under mild elution conditions (e.g., high salt, pH change). This is highly specific and achieves one-step purification.Why other methods are not ideal for one-step purification:• Cation exchange chromatography: separates by charge; not specific for histone-binding proteins.• Gel exclusion (SEC): separates by size; not specific.• Metal affinity (IMAC): works for His-tagged recombinant proteins, not native histone-binding proteins.Answer: Immuno-affinity chromatography (Option 2).