A protein with 1000 amino acids was tagged with GFP. The molecular weight of GFP is 26 KDa. What will be the most likely molecular weight of the…
A protein with 1000 amino acids was tagged with GFP. The molecular weight of GFP is 26 KDa. What will be the most likely molecular weight of the fused target protein?
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Correct Answer: Option B
Explanation
Average amino acid molecular weightDa (ranges from 75 Da for Gly to 186 Da for Trp; weighted average for a typical proteinDa). For a GFP-tagged protein: Protein (1000 AA):. GFP tag: 26 kDa (239 amino acids). Total fusion protein:. Note: for SDS-PAGE, the fusion protein would run as a single band at ~136 kDa. GFP does not significantly alter the migration pattern of large proteins but is easily detectable by fluorescence.