At what condition does the specific growth rate of the microorganisms decline in a constant volume fed-batch culture
At what condition does the specific growth rate of the microorganisms decline in a constant volume fed-batch culture
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Correct Answer: Option D
Explanation
In a constant-volume fed-batch culture, substrate feed rate(L/h) is fixed and feed substrate concentrationis constant. Substrate mass balance:. As fermentation proceeds, cell biomass(g/L) increases. With fixed, the substrate consumption termincreases with increasing. To maintain mass balance,falls → from Monod kinetics, decreasing→ decreasing. Therefore, as BIOMASS INCREASES in a constant-volume fed-batch culture, the specific growth rate DECLINES. This is a controlled growth strategy used to reduce oxygen demand, minimise acetate overflow in E. coli, and produce dense cultures.