For determining V_m and K_m of an enzyme catalyzed reaction, we often use a double reciprocal plot of 1/v versus 1/s. This is NOT the best method…
For determiningandof an enzyme catalyzed reaction, we often use a double reciprocal plot of 1/v versus 1/s. This is NOT the best method because:
Select an option to attempt
Correct Answer: Option D
Explanation
Lineweaver-Burk plot limitations (errors in velocity measurement):The Lineweaver-Burk (double-reciprocal) plot:Critical statistical problem:• When v is small (low substrate concentrations),is LARGE and subject to amplified errors.• A small absolute error in measuring v (e.g.,units) becomes a very large error in:- If,; an error ofin v gives(large spread).- If,; error becomes(small spread).• This NON-UNIFORM error distribution means points at low [S] (far left of plot) are unreliable and distort the linear regression.• Better alternatives: Eadie-Hofstee, Hanes-Woolf, or non-linear regression of v vs. [S] directly.Answer: Lineweaver-Burk amplifies errors in small velocity measurements; non-uniform error distribution makes linear fitting unreliable.