In an enzyme catalyzed reaction following Michaelis-Menten kinetics, both the enzyme amount and substrate (S) are doubled and we observe that the…
In an enzyme catalyzed reaction following Michaelis-Menten kinetics, both the enzyme amount and substrate (S) are doubled and we observe that the reaction rate is also doubled. From this, we can conclude that:
Select an option to attempt
Correct Answer: Option D
Explanation
Zero-order kinetics: doubling enzyme doubles rate:The Michaelis-Menten equation:If, then:This is ZERO-ORDER kinetics in [S]: the rate is independent of substrate concentration, depending only on.Consequences:• Doubling [S] at this regime has NO effect on rate (rate already maximal at).• Doubling enzyme concentration [E]ᵀ doubles→ doubles the rate.The observation that 'doubling S had no effect, but doubling enzyme doubled the rate' confirms we are at zero-order in S (i.e.,).Answer:(zero-order kinetics in S); rate is proportional to enzyme concentration, not substrate.