Sam was investigating impact of lactate dehydrogenase knockout on glycolytic pathway. What will be the net NADH production that he would expect in…
Sam was investigating impact of lactate dehydrogenase knockout on glycolytic pathway. What will be the net NADH production that he would expect in conversion of Glucose to pyruvate in this case?
Select an option to attempt
Correct Answer: Option A
Explanation
Glycolysis from glucose to pyruvate: the pathway is independent of LDH. NADH production occurs specifically at the GAPDH (glyceraldehyde-3-phosphate dehydrogenase) step:. This occurs TWICE per glucose molecule (since glucose is split into 2 triose phosphates). Net NADH from glycolysis:. LDH (lactate dehydrogenase) would normally oxidise NADH back to NAD(pyruvatelactate) to regenerate NADfor continued glycolysis. In a LDH KNOCKOUT, this regeneration is blocked, but NADH production (at GAPDH) during glycolysis itself still yields 2 NADH per glucose. The question asks specifically about NADH produced in glucose → pyruvate conversion = 2 NADH.