The enzyme-linked immunospot (ELISPOT) assay is highly sensitive because it can measure
The enzyme-linked immunospot (ELISPOT) assay is highly sensitive because it can measure
Select an option to attempt
Correct Answer: Option D
Explanation
Eremothecium ashbyi (= Ashbya gossypii), an ascomycete fungus, naturally overproduces riboflavin (Vitamin B2) by >1000-fold above its metabolic needs. The biosynthetic pathway produces GTP → 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione → riboflavin via 6,7-dimethyl-8-ribityllumazine. Industrial riboflavin fermentation (>50,000 tonnes/year) uses Ashbya gossypii or recombinant Bacillus subtilis. Riboflavin (B2) is the precursor to FMN (flavin mononucleotide) and FAD (flavin adenine dinucleotide), essential coenzymes in oxidative metabolism. Saccharomyces rouxii is used for soy sauce; S. cerevisiae for bread/beer/bioethanol; Candida utilis for SCP (single cell protein).