What will be the absorbance of a 1 mM solution of molecule X whose molar extinction coefficient is 1000 M^{-1}cm^{-1} & path length of the cuvette…
What will be the absorbance of a 1 mM solution of molecule X whose molar extinction coefficient is 1000& path length of the cuvette used is 1 cm?
Select an option to attempt
Correct Answer: Option A
Explanation
Beer-Lambert Law — absorbance calculation:The Beer-Lambert Law states:where:•= absorbance (dimensionless)•= molar extinction coefficient (M⁻¹cm⁻¹) = 1000 M⁻¹cm⁻¹•= concentration = 1 mM =M•= path length of cuvette = 1 cmCalculation:Note: The extinction coefficienthas units M⁻¹cm⁻¹, so units cancel:dimensionless.Answer: Absorbance = 1.0 (Option 1).