You are measuring blood glucose level by glucose oxidase method. Following are the absorbance values that you got for the standard and the test…
You are measuring blood glucose level by glucose oxidase method. Following are the absorbance values that you got for the standard and the test sample:
| Sample | Concentration | A505 |
|---|---|---|
| Standard 1 | 0.4 mmol/L | 0.120 |
| Standard 2 | 0.6 mmol/L | 0.180 |
| Standard 3 | 0.8 mmol/L | 0.240 |
| Unknown | ? | 0.210 |
Select an option to attempt
Correct Answer: Option B
Explanation
Glucose estimation by glucose oxidase method — linear interpolation from standard curve:Given data (from the table in the question):• Standard 2: [Glucose] = 0.6 mmol/L, A₅₀₅ = 0.180• Standard 3: [Glucose] = 0.8 mmol/L, A₅₀₅ = 0.240• Test sample: A₅₀₅ = 0.210 (falls between Standard 2 and 3)Using linear interpolation:Answer: 0.70 mM (Option 2).