You are preparing a 500 ml solution containing 50 mM Tris (FW 120) buffer, 10 mM NaCl (FW 58.5), 5 mM EDTA (supplied solution is 500 mM). The…
You are preparing a 500 ml solution containing 50 mM Tris (FW 120) buffer, 10 mM NaCl (FW 58.5), 5 mM EDTA (supplied solution is 500 mM). The quantity of each of the ingredients would be:
Select an option to attempt
Correct Answer: Option B
Explanation
Buffer preparation calculations for 500 mL:Using the formula:1. Tris (FW = 120 g/mol, 50 mM):2. NaCl (FW = 58.5 g/mol, 10 mM):3. EDTA (from 500 mM stock, need 5 mM in 500 mL):Using:Answer: 3.0 g Tris, 0.292 g NaCl, 5 mL EDTA (Option 2).