A diafiltration process is used to remove salts from a protein solution of 1L. After 1L of make-up buffer (without salts) has been added to this…
A diafiltration process is used to remove salts from a protein solution of 1L. After 1L of make-up buffer (without salts) has been added to this system, the residual salt concentration would be _____________ of the original.
Select an option to attempt
Correct Answer: Option B
Explanation
Continuous diafiltration: salt concentration after 1 volume exchange:Diafiltration replaces the solvent and removes small molecules (like salt) from a macromolecule solution by adding make-up buffer while simultaneously filtering.For continuous diafiltration, after addingdiavolumes (volumes of make-up buffer equal to the initial retentate volume):After 1 diavolume ():Interpretation: After adding 1 volume of buffer (1 diavolume), ~36.8% of the original salt remains.Comparison: After 2 diavolumes →; after 5 →.For efficient desalting, typically 3–5 diavolumes are used to achieve >95% removal.Answer: Salt fraction remaining after 1 diavolume.