Aqueous two phase partitioning (ATPS) is used for the recovery of an enzyme from the…

QuestionDBT JRF 2020Section BHardBioprocess Engineering

Aqueous two phase partitioning (ATPS) is used for the recovery of an enzyme from the cell free culture filtrate. On addition of PEG-2000 and dextran, the mixture separates into two phases with a partition coefficient for the enzyme as 4.2. The maximum possible enzyme recovery, when the volume ratio of the upper to lower phases is 5.0, will be

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Aqueous two phase partitioning (ATPS) is used for the recovery of an enzyme from the cell free culture filtrate. On addition of PEG-2000 and…

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