If an enzyme is NOT damaged during the process of entrapment in a bead, then internal pore diffusion will:
If an enzyme is NOT damaged during the process of entrapment in a bead, then internal pore diffusion will:
Select an option to attempt
Correct Answer: Option B
Explanation
Effect of enzyme entrapment on apparent Km and Vmax:Entrapment immobilises enzymes within a gel matrix (e.g., alginate, polyacrylamide beads). This creates a DIFFUSION BARRIER:Effect on kinetic parameters:1. ApparentINCREASES: Substrate must diffuse through the gel matrix to reach the enzyme. Higher [S] is needed externally to maintain adequate substrate concentration at the active site (overcomes the diffusion barrier). So more substrate is required for half-maximal rate →.2. ApparentDECREASES: The maximum rate is limited because not all enzyme active sites are accessible to substrate at high [S] due to diffusion limitations. Even saturating external [S] may not saturate all active sites within the bead.Bothandare affected by entrapment immobilisation.Answer: Entrapment increases apparent Km (diffusion barrier limits substrate access) and decreases apparent Vmax.