In a batch culture of cells following Monod Kinetics, the declining log phase lasts longer when:
In a batch culture of cells following Monod Kinetics, the declining log phase lasts longer when:
Select an option to attempt
Correct Answer: Option C
Explanation
Effect of substrate affinity constant Ks on growth kinetics:In the Monod equation:= substrate concentration at half-maximal growth rate (analogous to enzyme).Effect of a LARGE:• The organism has LOW affinity for the substrate (more substrate needed to reach half-maximal).• As substrate is consumed in the bioreactor,drops steeply as [S] falls.• This results in a longer, more pronounced decline in growth rate as substrate approaches exhaustion.• The 'declining log phase' (transition from log growth to stationary) is more extended whenis large.Effect of a SMALL:• HIGH affinity:remains nearuntil substrate is nearly completely exhausted.• Abrupt transition to stationary phase.Answer: Large→ low substrate affinity →drops faster as substrate depletes → longer declining log phase.