Short Chain variable fragments (ScFv) of antibody are composed of:
Short Chain variable fragments (ScFv) of antibody are composed of:
Select an option to attempt
Correct Answer: Option C
Explanation
Single-chain Fv (scFv) antibody structure:A single-chain Fv (scFv) antibody fragment consists of:•(variable heavy chain domain) connected by a flexible peptide linker to•(variable light chain domain)• Linker: typically 15 amino acids of (Gly₄Ser)₃ — flexible enough to allow proper domain folding• Molecular weight: ~28 kDa (about 1/6 of full IgG at ~150 kDa)• Retains full antigen-binding capacity (both CDR loops ofandcontribute to the paratope)Advantages of scFv:• Small size → better tissue penetration• Single gene product → easy to engineer and produce in bacteria• Basis for CAR-T cell constructs and bispecific antibodiesDisadvantages: No Fc region → short half-life, no complement activationAnswer: scFv =-linker-; full antigen-binding capacity, ~1/6 the size of IgG.