The role of DNA ligase in DNA replication is
The role of DNA ligase in DNA replication is
Select an option to attempt
Correct Answer: Option B
Explanation
DNA ligase plays a critical role in joining Okazaki fragments on the lagging strand during DNA replication. Specifically, after RNase H removes the RNA primer and DNA Polymerase I fills the gap with DNA, a nick remains between the 3'-OH of the newly synthesised DNA and the 5'-phosphate of the adjacent Okazaki fragment. DNA ligase seals this nick by forming a phosphodiester bond, using NAD⁺ (bacteria) or ATP (eukaryotes) as an energy source. This sealing step completes lagging strand synthesis and produces an uninterrupted daughter strand.