Which of the following is/are critical for genome replication?
Which of the following is/are critical for genome replication?
Select an option to attempt
Correct Answer: Option A
Explanation
DNA replication requires three classes of enzymes working in concert: (1) Helicase — unwinds the double helix by breaking hydrogen bonds between base pairs at the replication fork (e.g. DnaB in bacteria); (2) DNA Polymerase — synthesises the new strand in 5'→3' direction using each parental strand as template (requires a primer); (3) DNA Ligase — seals the nicks between Okazaki fragments on the lagging strand by forming phosphodiester bonds using NAD⁺ (bacteria) or ATP (eukaryotes) as cofactor. All three are indispensable for complete genome replication.