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Molecular Biology PYQs

41 previous year questions across 16 subtopics · answers and explanations included

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Showing 41 questions · page 1 of 3

1

In the Table given below, the column (A) lists three eukaryotic RNA Polymerases and column (B) lists group of genes they transcribe. Identify the correct match from the options below: [Table]

MediumRNA Polymerases & TranscriptionView answer

2

The motifs commonly found in the core promoter of eukaryotic protein coding genes are:

MediumEukaryotic Promoters & Transcription InitiationView answer

3

Eukaryotic RNA Polymerase II transcribes:

MediumRNA Polymerase II & Gene TranscriptionView answer

4

The most important step of an automated DNA sequencing reaction is

EasyView answer

5

What is the frequency with which a 4 bp cutter will cut the DNA, assuming random distribution of bases in the genome?

EasyView answer

6

Single stranded DNA can be separated from double stranded DNA efficiently using

HardView answer

7

A plasmid has three sites for EcoRI. When digested by this enzyme, incidentally partial digestion took place, though no uncut or nicked plasmid remained in the digestion mixture. The maximum number of bands that can be visualized in an agarose gel would be:

MediumRestriction Digestion & Gel ElectrophoresisView answer

8

For transcribing protein coding genes, the RNA Polymerase forms the preinitiation complex (PIC) in association with:

MediumTranscription Factors & PIC AssemblyView answer

9

Which of the following is/are critical for genome replication?

EasyView answer

10

The melting temperature ( t_m ) of DNA is higher when the content of:

MediumDNA Structure & Melting TemperatureView answer

11

The role of DNA ligase in DNA replication is

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12

Which of the following is a method of investigating the sequence specificity of DNbinding proteins in vitro?

MediumView answer

13

The genetic codon is a triplet and there are 64 codons. How many codons would be possible if the codon is a doublet?

EasyView answer

14

What will be the length of a DNA molecule containing 10 million bp?

MediumDNA Structure & Physical PropertiesView answer

15

Denaturation of DNA is a

MediumView answer

16

Which enzyme will digest RNA in a DNA-RNA Hybrid?

MediumNucleases & Nucleic Acid EnzymologyView answer

17

Which one of the following can be extended by Klenow enzyme upon addition of dNTPs and Mg2+ ?

HardView answer

18

Which one of the molecular processes utilize 2', 3' Dideoxy-ribonucleoside triphosphates?

MediumDNA Sequencing & ddNTPsView answer

19

Which of the following are NOT transcribed by RNA polymerase II?

HardView answer

20

TATA box involved in the initiation of transcription is located in the:

MediumPromoter Elements & TranscriptionView answer