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Molecular Biology PYQs

41 previous year questions across 16 subtopics · answers and explanations included

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Showing 25 questions · Medium · page 1 of 2

1

In the Table given below, the column (A) lists three eukaryotic RNA Polymerases and column (B) lists group of genes they transcribe. Identify the correct match from the options below: [Table]

MediumRNA Polymerases & TranscriptionView answer

2

The motifs commonly found in the core promoter of eukaryotic protein coding genes are:

MediumEukaryotic Promoters & Transcription InitiationView answer

3

Eukaryotic RNA Polymerase II transcribes:

MediumRNA Polymerase II & Gene TranscriptionView answer

4

A plasmid has three sites for EcoRI. When digested by this enzyme, incidentally partial digestion took place, though no uncut or nicked plasmid remained in the digestion mixture. The maximum number of bands that can be visualized in an agarose gel would be:

MediumRestriction Digestion & Gel ElectrophoresisView answer

5

For transcribing protein coding genes, the RNA Polymerase forms the preinitiation complex (PIC) in association with:

MediumTranscription Factors & PIC AssemblyView answer

6

The melting temperature ( t_m ) of DNA is higher when the content of:

MediumDNA Structure & Melting TemperatureView answer

7

Which of the following is a method of investigating the sequence specificity of DNbinding proteins in vitro?

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8

What will be the length of a DNA molecule containing 10 million bp?

MediumDNA Structure & Physical PropertiesView answer

9

Denaturation of DNA is a

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10

Which enzyme will digest RNA in a DNA-RNA Hybrid?

MediumNucleases & Nucleic Acid EnzymologyView answer

11

Which one of the molecular processes utilize 2', 3' Dideoxy-ribonucleoside triphosphates?

MediumDNA Sequencing & ddNTPsView answer

12

TATA box involved in the initiation of transcription is located in the:

MediumPromoter Elements & TranscriptionView answer

13

Met-Ile-Val-His-Tyr was the sequence of a hypothetical peptide. Assuming that there are two possible codons each for His, Val and Tyr, one possible codon for Met, and four possible codons for Ile, the number of possible nucleotide sequences coding for this…

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14

Which one of the following markers is NOT routinely used for DNA fingerprinting?

MediumDNA Fingerprinting & Polymorphism MarkersView answer

15

DNA isolated from four unidentified species of bacteria - A, B, C and D has been estimated to have 38%, 26%, 24% and 12% thymine, respectively. One of these four species was isolated from a hot spring (64°C). Identify the candidate which is most likely to be…

MediumDNA Base Composition & GC ContentView answer

16

Given below are two statements: Statement I: Polycistronic mRNAs are found in archaea and bacteria. Statement II: Plastid genes in eukaryotes have only mono-cistronic mRNA. In the light of the above statements, choose the most appropriate answer from the…

MediummRNA Structure - Polycistronic vs MonocistronicView answer

17

Which one of the following enzymes help the DNA to overcome the tension generated by the unwinding during replication?

MediumDNA Replication - TopoisomerasesView answer

18

Which one of the following types of mutations can lead to a major change in the encoded protein? A. Insertion of a single nucleotide near the end of the coding sequence. B. Removal of a single nucleotide from the beginning of the coding sequence. C. Deletion…

MediumMutations & Reading Frame ShiftsView answer

19

In the cloverleaf structure of tRNA, the cognate amino acid is attached at

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20

Which of the following is NOT a feature of bacterial DNA replication?

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