The melting temperature ( t_m ) of DNA is higher when the content of:
The melting temperature () of DNA is higher when the content of:
Select an option to attempt
Correct Answer: Option B
Explanation
DNA melting temperature () and G:C content:The melting temperature () is the temperature at which 50% of double-stranded DNA is denatured.Approximate formula for short sequences:(in °C, for oligonucleotides)Reason G:C raises:• G:C base pair: stabilised by 3 hydrogen bonds• A:T base pair: stabilised by only 2 hydrogen bondsMore G:C → more hydrogen bonds → greater thermal energy required to separate strands → higher.Answer: G:C base pair content is higher (Option 2).