For transcribing protein coding genes, the RNA Polymerase forms the preinitiation complex (PIC) in association with:
For transcribing protein coding genes, the RNA Polymerase forms the preinitiation complex (PIC) in association with:
Select an option to attempt
Correct Answer: Option C
Explanation
RNA Pol II Preinitiation Complex (PIC) formation:For RNA Pol II to begin transcription, it must assemble the PIC in a defined order:1. TFIID (containing TBP): Binds the TATA box, nucleating PIC assembly.2. TFIIA: Stabilises TFIID-TATA interaction.3. TFIIB: Bridges TFIID and Pol II; required for Pol II recruitment.4. TFIIF: Brings RNA Pol II to the promoter; stabilises the Pol II–TFIIB interaction.5. TFIIE and TFIIH: Join later; TFIIH has helicase activity to unwind DNA at the TSS and CDK7 kinase activity to phosphorylate the CTD of Pol II, triggering promoter clearance and elongation.The minimal functional complex includes TFIID, TFIIF, and TFIIH (Option 3). TFIIG and TFIIC do not exist in Pol II transcription.Answer: TFIIH, TFIIF, TFIID (Option 3).