Single stranded DNA can be separated from double stranded DNA efficiently using
Single stranded DNA can be separated from double stranded DNA efficiently using
Select an option to attempt
Correct Answer: Option D
Explanation
Hydroxyapatitechromatography exploits differential adsorption of nucleic acids: dsDNA binds via both phosphate groups (to Ca²⁺ sites) and bases (to PO₄³⁻ sites) forming a stronger interaction, while ssDNA binds primarily through phosphate groups only. At low phosphate buffer concentrations (e.g. 10 mM NaH₂PO₄), ssDNA elutes while dsDNA remains bound. Increasing phosphate concentration (e.g. 400 mM) then elutes dsDNA. This differential affinity enables efficient separation of ssDNA from dsDNA, and is also used to separate RNA from DNA and dsDNA from ssDNA–dsDNA hybrids.