A covalently closed circular DNA was in a relaxed state in water at 30 °C. What will happen if the water temperature increases to 60 °C or decreases…

Select an option to attempt

1. A
   DNA will be relaxed at 60 °C and positively supercoiled at 10 °C
2. B
   DNA will undergo positive supercoiling at 60 °C and negative supercoiling at 10 °C
3. C
   DNA will be negatively supercoiled at both 60 °C and 10 °C
4. D
   DNA will undergo negative supercoiling at 60 °C and positive supercoiling at 10 °C