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Biochemistry PYQs

105 previous year questions across 53 subtopics · answers and explanations included

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Showing 105 questions · page 3 of 6

41

What will be the final concentration of the solute when 10 ml of its 10 M solution is mixed with 990 ml solvent?

MediumSolution Dilutions & ConcentrationView answer

42

How many chiral centers are there in each of these two molecules? [Image]

MediumStereochemistry & Chiral CentersView answer

43

The CO-NH moiety between two C-alpha atoms constitutes the Peptide bond. The measured bond lengths (Angstroms) are: C(alpha)-CO (1.51); C=O (1.24); C-N(1.33); N-C(alpha)1.46. In which of these, there is a decrease from the expected bond length due to partial…

MediumProtein Structure - Peptide BondView answer

44

Which one of these is a metal containing Vitamin?

MediumVitamins & CofactorsView answer

45

How many different kinds of post translational modifications are present in this peptide? [Image]

MediumPost-Translational ModificationsView answer

46

Given the molecular structure of glucose, the molecular formula and molecular weight of maltose will be: [Image]

MediumCarbohydrate Structure & Molecular FormulaView answer

47

What will be the absorbance of a 1 mM solution of molecule X whose molar extinction coefficient is 1000 M^{-1}cm^{-1} & path length of the cuvette used is 1 cm?

MediumSpectrophotometry - Beer-Lambert LawView answer

48

Amide bonds between two amino acids (as shown below) are generally in trans-conformation. The atoms are labeled as A1 through A6. The angle measured for defining the trans-conformation of the amide bond is the angle between: [Image]

MediumProtein Structure - Peptide Bond ConformationView answer

49

A protein with 10 lysines and 2 arginine residues was digested with trypsin and the peptide mixture was resolved on an SDS-PAGE. After silver staining, 10 bands were detected instead of 13. Select the plausible reasons from the following: A. Some of the…

MediumProteolysis & Trypsin DigestionView answer

50

The probe used to analyse glycoproteins is

MediumView answer

51

15 to 30% of collagen residues are 4-hydroxyproline residues (Hyp). When rats are fed with ¹⁴C labeled Hyp, the collagen produced by them is not radioactive, but when they are fed with ¹⁴C labeled Proline, the collagen produced is radioactive. What is the…

MediumCollagen & Hydroxyproline BiosynthesisView answer

52

A protein completely unfolds in 8 M Urea. The stability of the folded state in water is calculated to be 8 kCal/mole at 25°C. What is the likely ΔG of the following reaction in 8 M Urea? A(native) → A(unfolded) Choose the correct answer :

MediumProtein Folding & ThermodynamicsView answer

53

A following reaction is occurring in a test tube: A + B ⇌ C 100 nM solution of A is incubated with an equal volume of 100 nM solution of B and left at 25°C till the equilibrium is reached. Finally, when the concentration of A is measured, it is found to be…

MediumChemical Equilibrium & Reaction KineticsView answer

54

A protein sample isolated from natural source was electrophoresed on a non-reducing SDS-PAGE. It migrated as a band corresponding to 60 kDa. When the same protein was electrophoresed on a native-PAGE its migration corresponded to a 120 kDa marker protein.…

MediumProtein Characterization - SDS-PAGE & Native PAGEView answer

55

Which histone is NOT a part of the nucleosomes?

EasyView answer

56

The energy landscape or the energy of a reaction: A ⇌ B Along its reaction coordinate follows the following pattern: [Image] Which one of the following statements are true?

MediumEnzyme Reaction Coordinate & Energy LandscapeView answer

57

If four atoms A, B, C and D are connected linearly and there is rotation possible along bond BC, dihedral angle on this bond is described as

HardView answer

58

A laboratory is trying to purify a Histone-binding protein from its native source. Which one of the following purification techniques should be used for one-step purification of the protein?

MediumProtein Purification TechniquesView answer

59

A 1 M Tris-HCl (pH 8.0) solution was diluted 10 fold. What will be the pH of the resulting solution?

MediumBuffer Chemistry & pHView answer

60

When ice absorbs latent heat at a constant pressure to melt from solid to liquid state at constant temperature, which of the following changes are happening? A. Entropy of the system is increasing. B. Cp (heat capacity at constant pressure) of the system is…

MediumThermodynamics - Phase TransitionsView answer