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Biochemistry PYQs

105 previous year questions across 53 subtopics · answers and explanations included

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Showing 70 questions · Medium · page 3 of 4

41

A protein sample isolated from natural source was electrophoresed on a non-reducing SDS-PAGE. It migrated as a band corresponding to 60 kDa. When the same protein was electrophoresed on a native-PAGE its migration corresponded to a 120 kDa marker protein.…

MediumProtein Characterization - SDS-PAGE & Native PAGEView answer

42

The energy landscape or the energy of a reaction: A ⇌ B Along its reaction coordinate follows the following pattern: [Image] Which one of the following statements are true?

MediumEnzyme Reaction Coordinate & Energy LandscapeView answer

43

A laboratory is trying to purify a Histone-binding protein from its native source. Which one of the following purification techniques should be used for one-step purification of the protein?

MediumProtein Purification TechniquesView answer

44

A 1 M Tris-HCl (pH 8.0) solution was diluted 10 fold. What will be the pH of the resulting solution?

MediumBuffer Chemistry & pHView answer

45

When ice absorbs latent heat at a constant pressure to melt from solid to liquid state at constant temperature, which of the following changes are happening? A. Entropy of the system is increasing. B. Cp (heat capacity at constant pressure) of the system is…

MediumThermodynamics - Phase TransitionsView answer

46

Which of the following are the sulfur containing amino acids? i. Methionine ii. Histidine iii. Leucine iv. Cysteine

MediumAmino Acid Structure & PropertiesView answer

47

Arrange the following chemical bonds/interactions in the order of their strengths in vacuum - Hydrogen bond, Covalent bond, van der Waals attraction and Ionic bond:

MediumChemical Bonds & Intermolecular ForcesView answer

48

Match the items in left column with those in the right column: [Table] Choose the correct answer from the options given below :

MediumBiochemical Concepts - Match the FollowingView answer

49

Which one of the following properties of naturally occurring D-amino acids is INCORRECT:

MediumAmino Acid Properties - D & L FormsView answer

50

Which one of the following will NOT change upon adding an enzyme to a chemical reaction or changing the concentration of reactants or products? A. ΔG° B. K_eq C. ΔG

MediumThermodynamics & Reaction ParametersView answer

51

In the DNA helix, the GC and AT base pairs

MediumView answer

52

A protein with 1000 amino acids was tagged with GFP. The molecular weight of GFP is 26 KDa. What will be the most likely molecular weight of the fused target protein?

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53

The location of a proteins in cells can be studied using

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54

Given below are two statements one is labelled as Assertion A and the other is labelled as Reason R: Assertion A: In size exclusion chromatography, larger proteins elute earlier and smaller proteins elute later. Reason R: When matrices like sephadex are…

MediumSize Exclusion ChromatographyView answer

55

In an enzyme catalyzed reaction following Michaelis-Menten kinetics, both the enzyme amount and substrate (S) are doubled and we observe that the reaction rate is also doubled. From this, we can conclude that:

MediumEnzyme Kinetics - Substrate ConcentrationView answer

56

The entropy of a growing cell does NOT keep increasing continuously because:

MediumThermodynamics of Living SystemsView answer

57

For determining V_m and K_m of an enzyme catalyzed reaction, we often use a double reciprocal plot of 1/v versus 1/s. This is NOT the best method because:

MediumEnzyme Kinetics - Lineweaver-Burk PlotView answer

58

Which one of the following is NOT a food rich in Vitamin A?

MediumVitamins & NutritionView answer

59

The red color of beetroot is due to the presence of:

MediumPlant Pigments - BetalainsView answer

60

A protein sequence has twenty consecutive hydrophobic amino acids followed by a few hydrophilic residues and this pattern repeats itself five to seven times. Which kind of a protein is described here?

MediumMembrane Proteins & Hydrophobic SegmentsView answer