DBT JRF 2022

Official DBT JRF 2022 previous year paper for practice and preparation.

All questions with answers

1. Q1In the Table given below, the column (A) lists three eukaryotic RNA Polymerases and column (B) lists group of genes they transcribe. Identify the correct…
2. Q2500 mg of a drug (Molecular weight 100) was intravenously injected into an individual having a blood volume of 5 liter. The drug is neither absorbed by the…
3. Q3The motifs commonly found in the core promoter of eukaryotic protein coding genes are:
4. Q4The cytosine residues in the DNA are methylated at specific di- or trinucleotide sequences. Which among these methylation motifs are found predominantly in…
5. Q5While measuring the potassium concentration in a drug formulation, the technologist took 10 µl of the sample and mixed it with 90 µl of test buffer. From the…
6. Q6You are given a 5 mM solution of sucrose. If you hydrolyse it by adding equal volume of 10 mM HCl, what would be the final concentration of glucose and…
7. Q7You are measuring blood glucose level by glucose oxidase method. Following are the absorbance values that you got for the standard and the test sample: [Table]
8. Q8The introduction of the nonpolar molecule (toluene) into water results in:
9. Q9Eukaryotic RNA Polymerase II transcribes:
10. Q10You are preparing a 500 ml solution containing 50 mM Tris (FW 120) buffer, 10 mM NaCl (FW 58.5), 5 mM EDTA (supplied solution is 500 mM). The quantity of each…
11. Q11In eukaryotes, nucleosomes comprise of which one of the following histones?
12. Q12Match the items in List I with the items in List II: [Table] Choose the correct answer from the options given below:
13. Q13Which one of the following statements is true with respect to optical activity?
14. Q14Which one of the following compound(s) is/are NOT the carbon source of acetyl Co-A?
15. Q15How many rectangles are there in the diagram? [Image]
16. Q16Find the next image in the series. [Image]
17. Q17How many words can be formed with the letters of the word 'PATALIPUTRA'?
18. Q18A ladder leaning against a wall is making an angle of 60° with the ground. If the length of the ladder is 18 m, find the distance of the foot of the ladder…
19. Q19The line y = 2x from x = 0 to 1 is rotated around the y-axis. The volume generated is:
20. Q20The function y = f(x) = x^2 - 6 is plotted along with its inverse function y = f^{-1}(x) , where essentially the x & y axis are interchanged. These two curves…
21. Q21The probability that 1st of January is a Sunday in at least one of the 3 randomly chosen years is given by:
22. Q22An aircraft circumnavigates the earth 10 km above the earth's surface. If it completes one full circle, the distance covered will be _____ greater than the…
23. Q23A growing bacterial culture doubles in 1 hr; the increase in cell mass in 30 minutes will be:
24. Q24Two random numbers are generated between zero and one and added. The probability that the sum of these two numbers will be between 0.5 and 1.5 is:
25. Q25If 2^x = 3^y = 6^z = K , then the relationship between x, y, and z is given by:
26. Q26A plasmid has three sites for EcoRI. When digested by this enzyme, incidentally partial digestion took place, though no uncut or nicked plasmid remained in…
27. Q27An enzyme is governed by Michaelis-Menten Kinetics. The apparent K_M increases four fold due to the addition of inhibitor but the reaction rate declines by…
28. Q28A culture contains two bacterial populations 'X' and 'Y' in equal proportion. On dilution plating only two colonies were obtained due to excessive dilution.…
29. Q29For transcribing protein coding genes, the RNA Polymerase forms the preinitiation complex (PIC) in association with:
30. Q30During signal transduction, one molecule of phosphatidylinositol 4,5-bisphosphate (PIP2) is cleaved into one molecule each of inositol triphosphate (IP3) and…
31. Q31Identify the INCORRECT statement for a competitive reaction.
32. Q32The melting temperature ( t_m ) of DNA is higher when the content of:
33. Q33Gap junctions are absent in:
34. Q34The enzyme that links carbon and nitrogen metabolism is:
35. Q35The cytosine residues in the DNA are methylated at specific di- or tri-nucleotide sequences. Which among these methylation motifs is predominantly found in…
36. Q36Which one of the following acts as a linker histone?
37. Q37If you mixed 20 mL of 50 mM sodium acetate (NaOAc) with 30 mL of 25 mM glucose, what would be the concentration of each in the final solution?
38. Q38If the numerator of a fraction is increased by 15% and its denominator is decreased by 8%, the new fraction becomes 15/16. Find the original fraction.
39. Q39Identify the sum of a+b+c, provided the total of each row and each column is same. [Table]
40. Q40Add one more element to the given number series: 71, 76, 69, 74, 67, 72:
41. Q41If 48 men can do a piece of work in 25 hours, how much time will it take for 15 men to finish the same task?
42. Q42A 100 m long train is running at a speed of 68 km/h. A man is also running in the same direction with a speed of 8 km/h. In what time will the train overtake…
43. Q43What will be the length of a DNA molecule containing 10 million bp?
44. Q44If 8% of x = 4% of y, then 20% of x is?
45. Q45If x = y^a , y = z^b , z = x^c , find the value of abc.
46. Q46Find the number of triangles in the given figure: [Image]
47. Q47Which one of these cubes (labelled A - D) will have the highest surface to volume ratio? The length of sides of each cube are given in cm. [Image] [Image]…
48. Q48What is the molecular formula for the structure given below? [Image]
49. Q49In Reverse Phase chromatography of a mixture of protein and sodium chloride:
50. Q50What is the concentration of D_2O in heavy water (density 1.11 g/ml)?
51. Q1Green Fluorescent Protein (GFP) emits green light due to the presence of a:
52. Q2What is the molarity of 100 g Glycine (75 Daltons) dissolved in 1 liter water?
53. Q3What will be the molecular weight of the tri-peptide Trp-Gly-His? Molecular weights of Trp, Gly, and His are 204.2, 75.07, and 155.2 respectively.
54. Q4How many times is the Ostrich egg bigger than the mitochondrion? [Image]
55. Q5What, if any, is the difference between the molecular weights of molecule A (left) vs molecule B (right)? [Image]
56. Q6What may be the simplest and most inexpensive way to know that the product is formed in the reaction given below? [Image]
57. Q7What will be the final concentration of the solute when 10 ml of its 10 M solution is mixed with 990 ml solvent?
58. Q8Which enzyme will digest RNA in a DNA-RNA Hybrid?
59. Q9How many chiral centers are there in each of these two molecules? [Image]
60. Q10The CO-NH moiety between two C-alpha atoms constitutes the Peptide bond. The measured bond lengths (Angstroms) are: C(alpha)-CO (1.51); C=O (1.24); C-N(1.33);…
61. Q11Which one of these is a metal containing Vitamin?
62. Q12How many different kinds of post translational modifications are present in this peptide? [Image]
63. Q13Given the molecular structure of glucose, the molecular formula and molecular weight of maltose will be: [Image]
64. Q14What will be the absorbance of a 1 mM solution of molecule X whose molar extinction coefficient is 1000 M^{-1}cm^{-1} & path length of the cuvette used is 1 cm?
65. Q15Amide bonds between two amino acids (as shown below) are generally in trans-conformation. The atoms are labeled as A1 through A6. The angle measured for…
66. Q16Which one of the molecular processes utilize 2', 3' Dideoxy-ribonucleoside triphosphates?
67. Q17Proteins are glycosylated in the endoplasmic reticulum. In the final structures, the glycan moieties are typically present:
68. Q18A protein with 10 lysines and 2 arginine residues was digested with trypsin and the peptide mixture was resolved on an SDS-PAGE. After silver staining, 10…
69. Q1915 to 30% of collagen residues are 4-hydroxyproline residues (Hyp). When rats are fed with ¹⁴C labeled Hyp, the collagen produced by them is not radioactive,…
70. Q20A protein completely unfolds in 8 M Urea. The stability of the folded state in water is calculated to be 8 kCal/mole at 25°C. What is the likely ΔG of the…
71. Q21A following reaction is occurring in a test tube: A + B ⇌ C 100 nM solution of A is incubated with an equal volume of 100 nM solution of B and left at 25°C…
72. Q22A protein sample isolated from natural source was electrophoresed on a non-reducing SDS-PAGE. It migrated as a band corresponding to 60 kDa. When the same…
73. Q23The energy landscape or the energy of a reaction: A ⇌ B Along its reaction coordinate follows the following pattern: [Image] Which one of the following…
74. Q24A laboratory is trying to purify a Histone-binding protein from its native source. Which one of the following purification techniques should be used for…
75. Q25A 1 M Tris-HCl (pH 8.0) solution was diluted 10 fold. What will be the pH of the resulting solution?
76. Q26When ice absorbs latent heat at a constant pressure to melt from solid to liquid state at constant temperature, which of the following changes are happening?…
77. Q27The term Dysbiosis is associated with:
78. Q28Which one of the immunodeficiency is caused by a mutation in BTK gene:
79. Q29Which one of the following Immunoglobulins cause type 1 allergic reaction?
80. Q30TATA box involved in the initiation of transcription is located in the:
81. Q31Given below are two statements: Statement I: MacConkey agar is a media used for recovery of Gram positive bacteria from mixed bacterial culture. Statement II:…
82. Q32Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R Assertion A: Mycoplasma is a bacteria without cell wall,…
83. Q33Which one of the following markers is NOT routinely used for DNA fingerprinting?
84. Q34A specialized histone associated with the centromeric region of the chromosome is:
85. Q35Which of the following are the sulfur containing amino acids? i. Methionine ii. Histidine iii. Leucine iv. Cysteine
86. Q36DNA isolated from four unidentified species of bacteria - A, B, C and D has been estimated to have 38%, 26%, 24% and 12% thymine, respectively. One of these…
87. Q37Given below are two statements: Statement I: Polycistronic mRNAs are found in archaea and bacteria. Statement II: Plastid genes in eukaryotes have only…
88. Q38A conventional light microscope allows us to magnify cells up to 1000 times and to resolve details as small as ______________.
89. Q39Arrange the following chemical bonds/interactions in the order of their strengths in vacuum - Hydrogen bond, Covalent bond, van der Waals attraction and Ionic…
90. Q40Which one of the following is NOT a major checkpoint in the cell division cycle of eukaryotic cell?
91. Q41Which one of the following CANNOT be used for progressive global multiple sequence alignment?
92. Q42Match the items in left column with those in the right column: [Table] Choose the correct answer from the options given below :
93. Q43Given below are two statements: one is labelled as Assertion A and the other is labelled as Reason R. Assertion A: Histones bind to DNA with high affinity.…
94. Q44The color of emitted light of Quantum dots used in fluorescence microscopy depend on:
95. Q45Which one of the following properties of naturally occurring D-amino acids is INCORRECT:
96. Q46Which one of the following will NOT change upon adding an enzyme to a chemical reaction or changing the concentration of reactants or products? A. ΔG° B. K_eq…
97. Q47Which of the following statements are correct? A. Lipid bilayer lacking proteins is highly impermeable to all the charged molecules. B. Channels have specific…
98. Q48Which one of the following enzymes help the DNA to overcome the tension generated by the unwinding during replication?
99. Q49Which one of the following types of mutations can lead to a major change in the encoded protein? A. Insertion of a single nucleotide near the end of the…
100. Q50Which one of the following organisms is NOT an obligate intracellular parasite and, therefore, can grow on bacteriological media?